Figure 6.

Local treatment of N46 inhibits acute nociceptive response elicited by intraplantar injection of capsaicin. Mechanical thresholds for paw withdrawal were determined at designated time points after injecting either 10 μg capsaicin, 10 nmol N46, or 10 nmol N46 immediately followed by 10 μg capsaicin. (A) Injecting both N46 and capsaicin significantly increased mechanical threshold measured at 5, 10, 30, and 60 minutes later when compared to capsaicin treatment only (*P = 0.0059, <0.0001, 0.0002 and 0.0049, respectively; n = 15 in each group). (B) Injection of both N46 and capsaicin also produced a significant increase in withdrawal latency 5 minutes later at postinjection 5 minutes compared to the capsaicin alone (*P < 0.0001). Data represent mean ± SEM.