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Effect of HU on apoptosis in mSSCs. After HU (0, 0.225, 0.45 and 0.9mM) treatment for 24 h, mSSCs that stained (A) Annexin V, (B) PI and (C) TUNEL were analyzed using FACS. Note upper lines; isotype controls, the broad peak of Annexin-V-Cy3-positive, PI-positive or TUNEL-positive fluorescent cells induced apoptosis. All data represent mean ± SD. *p < 0.05, **p < 0.01 or ***p < 0.001 versus vehicle control.
