Column choice is important for resolving components in a sample for SEC-SAXS.
Here, bovine serum albumin, that consists of monomers, dimers and higher
oligomers, is separated using three different column matrices. The small S200
5/150 column shows a level of monomer separation (highest peak) from the
oligomers, but there is a chance that these monomers are not completely
separated from the other self-associated states. The S200 Increase produces well
resolved peaks enabling separation of the monomer and dimer components for SAXS
analysis.