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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Protoc. 2016 Oct 6;11(11):2122–2153. doi: 10.1038/nprot.2016.113

Figure 14. Resolving power of SEC columns for SEC-SAXS.

Figure 14

Column choice is important for resolving components in a sample for SEC-SAXS. Here, bovine serum albumin, that consists of monomers, dimers and higher oligomers, is separated using three different column matrices. The small S200 5/150 column shows a level of monomer separation (highest peak) from the oligomers, but there is a chance that these monomers are not completely separated from the other self-associated states. The S200 Increase produces well resolved peaks enabling separation of the monomer and dimer components for SAXS analysis.