Figure 2.
The effect of HN on NMDA-induced cell viability reduction of rat cortical neurons.
Notes: Rat cortical neurons were treated with NMDA (100 μmol/L). HN was added at concentrations of 0, 0.001, 0.01, 0.1, 1, 10, and 100 μmol/L. Cell viability was measured using the MTT assay. Each point indicates the average of 5 wells from 2 separate experiments. In order to show the point of “0” on a log curve, we plotted “0” using a very small number, namely, 10−6. Data are expressed as the percentage of cell viability of the control (mean ± SD).
Abbreviations: HN, humanin; MTT, dimethylthiazolyldiphenyl tetrazolium bro mide; NMDA, N-methyl-D-aspartate; SD, standard deviation.