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Osteogenic (OB) and adipogenic (AD) differentiation of BM-MSCs was directed by culture surface. (A) OB differentiation of the BM-MSCs was visualized using Alizarin Red staining for calcification and AD differentiation was visualized using Oil Red O staining for lipid droplets. (B) Quantification of pixel staining, as a measure of differentiation efficiency, was performed on 15 randomly-selected areas for each substrate. *P < 0.05, vs. TCP; âĄP < 0.05, vs. BM-ECM.
