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. 2017 Apr 19;12:3235–3252. doi: 10.2147/IJN.S122945

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© 2017 Das et al. This work is published and licensed by Dove Medical Press Limited

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Cytocompatibility of CNT films.

Notes: (A) Cell proliferation assay (MTT assay) of canine MSCs cultured on control and different CNT films up to Day 6. The symbols * and ^# indicate significance (P<0.05) with respect to control and between the films, respectively, on a particular day. The results are the mean ± standard error of the mean of triplicate experiments. (B) Relative expression of apoptosis-associated genes in canine MSCs cultured on control and different CNT films. Results normalized to GAPDH relative to control. Dashed line indicates values of target genes in control conditions. The symbols * and ^# indicate significance (P<0.05) with respect to control and between the films, respectively, for a target gene. (C) Flow cytometry assay of early apoptotic, late apoptotic, and necrotic canine MSCs (percentage of cells) cultured on control and different CNT films for a period up to 6 days. The symbols * and ^# indicate significance (P<0.05) with respect to control and between the films, respectively, at an individual stage of apoptosis on a particular day.

Abbreviations: CNT, carbon nanotube; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MSC, mesenchymal stem cell; MTT, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; MWCNT, multiwalled CNT; PEG, polyethylene glycol; SWCNT, single-walled CNT.