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. 2017 Apr 24;12(4):e0174704. doi: 10.1371/journal.pone.0174704

Fig 4. The transcript changes (folds) of flagella-related genes between KJ and KJΔBC cells by transcriptome analysis.

Fig 4

Total mRNA was extracted from KJ and KJΔBC logarithmic-phase cultures. The ribosomal RNA (rRNA) depletion, adapter-ligated cDNA library construction and enrichment, and cDNA sequencing were performed as described in Materials and Methods. Transcript changes (folds) of a gene is expressed as the transcript in KJΔBC relative to the transcript in wild-type KJ (KJΔBC/KJ). Navy blue color indicates the transcript change of the gene is greater than or equal to 3. Blue color indicates the transcript change of the gene is less than 3 and greater than 2. Light blue color indicates the transcript change of the gene is less than 2 and greater than 1. (A) The genomic organizations of the flagella-related genes. Based on the transcriptome assay, the flagella-related genes, upregulated in case of creBC inactivation, are located in three clusters, Smlt0561-0562, Smlt2265-2290, and Smlt2302-2321. The orientation of gene is indicated by the arrow. (B) Schematic diagram of bacterial flagellum. The flagellum consists of the basal body, the hook, and the filament. The composition proteins are labelled. OM, outer membrane; PG, peptidoglycan layer; IM, inner membrane.