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. 2017 Apr 24;12(4):e0173738. doi: 10.1371/journal.pone.0173738

Fig 4. RSV plaques detected in HeLa cultures co-cultured with cord blood mononucleocytes (CBM) matured with PHA and treated with soluble nitic oxide (NO) and palivizumab (PZB).

Fig 4

Cryopreserved cord blood samples were treated with PHA, ‘PHA with NO’ or ‘PHA with NO and PZB’ for 24hrs, then added to HeLa cells grown to 70% confluence. Significantly more RSV was detected in all HeLa monolayers co-cultured with CBMs regardless of treatment compared to HeLa monolayers alone (p<0.01). Non-treated CBMs co-cultured with HeLa cells had less RSV plaques then similar cultures treated with PHA and PHA with NO. While RSV was detected in co-cultures treated with ‘PHA, NO and PZB’ this was not significantly different to non-treated CBM co-cultures (Mann-Whitney U analysis, p = 0.193; n = 11 samples for all treatments).