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. 2017 Apr 24;12(4):e0175894. doi: 10.1371/journal.pone.0175894

Fig 4. Age-associated changes in AmBRP and Synapsin levels in the central brain of Apis mellifera.

Fig 4

a Representative Western blot of central brains from 1-, 8-, 15-, 29- and 43-day-old bees. Shown are AmBRP proteins migrating around 220 kDa, Synapsin proteins around 70 kDa and the α-Tubulin band around 60 kDa. b-c Results from the quantitative Western blot analysis using central brain homogenate from 1-, 8-, 15-, 29- and 43-day-old bees. b The level of AmBRP in the central brain is increased in the group of 15-day-old worker bees compared with 1-day-old bees. c The level of Synapsin is increased in the group of 15-, 29- and 43-day-old worker bees compared with 1-day-old bees. Box blots show median, 25% and 75% quartiles and value range (min-max). (*) Significant differences (p < 0.05) detected with Mann-Whitney U test after Kruskal Wallis ANOVA.