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. 2017 Apr 24;12(4):e0175894. doi: 10.1371/journal.pone.0175894

Table 1. Primary antibodies used in this study.

Name of primary antibody Immunogen Manufacturer, Cat. No., Species raised in, Mono-/Polyclonal Antibody dilution
Anti-BRPlast200 Last 200 amino acids of large D. melanogaster Bruchpilot isoforms (e.g. amino acids 1540–1740 of isoform D, protein accession number: NP_724796) Prof. Dr. Sigrist, rabbit, polyclonal (Ullrich et al., 2015) Western blot: 1:6,000 Immuno-histochemistry: 1:500
Anti-BRPD2 D. melanogaster Bruchpilot domain D2 (amino acids 268–617) Prof. Dr. Sigrist, rabbit, polyclonal (Fouquet et al., 2009) Western blot: 1:20,000
Anti-α-Tubulin mouse mAb (DM1A) Native chicken brain microtubules Calbiochem, Sandhausen, Germany, #CP06, RRID: AB_212802, mouse, monoclonal Western blot: 1:3,000
Anti-SYNORF1 D. melanogaster synapsin Glutathione S-transferase fusion protein, epitope: 341 LFGGMEVCGL 350 DSHB, Iowa City, IA, USA, Anti-SYNORF1, #3C11, RRID: AB_2313867, mouse, monoclonal Western blot: 1:1,500 Immuno-histochemistry: 1:100