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. 2017 Apr 12;13(4):e1006301. doi: 10.1371/journal.ppat.1006301

Fig 1. Expression patterns of MgGPP in Meloidogyne graminicola.

Fig 1

(A) Schematic representation of M. graminicola pre-J2. (B) Localization of MgGPP in the subventral esophageal glands of M. graminicola pre-J2s by in situ hybridization. Fixed nematodes were hybridized with (left) sense and (right) antisense cDNA probes from MgGPP. Scale bars, 10 μm. (C) The developmental expression pattern of MgGPP by RT-qPCR analysis in five different life stages of M. graminicola. The fold change values were calculated using the 2-ΔΔCT method and presented as the change in mRNA level at various nematode developmental stages relative to that of the egg stage. The data shown are the means of three repeats plus standard deviation (SD), and three independent experiments were performed with similar results. dpi, days post-infection; pre-J2, pre-parasitic second-stage juvenile; par-J2, par-J3 and par-J4, parasitic second-, third- and fourth-stage juveniles, respectively.