Table 1.
Primary antibodies and their source.
| Antibody against | Description | Specifity/Immunogen | Used conc. | Catalog Nr., Manufacturer |
|---|---|---|---|---|
| Bromo-deoxyuridine | Mouse monoclonal | Detects BrdU incorporated into DNA as well as bromouridine incorporated into RNA | 1:200 | A21300, Invitrogen |
| Centromere Protein | Human | Derived from human CREST patient serum | 1:50 | 15–234, Antibodies Inc. |
| CHK2 (phospho T68) | Rabbit polyclonal | Epitope around the phosphorylation site of Threonine 68 (VSTpQE) of human Chk2 | 1:100 | ab38461, Abcam |
| Fibrillarin | Mouse monoclonal | Corresponding to Saccharomyces cerevisiae Fibrillarin | 1:50 | ab4566, Abcam |
| Histone H3K4me3 | Mouse monoclonal | Synthetic peptide corresponding to Human Histone H3 aa 1–100 (tri methyl K4) conjugated to KLH | 1:50 | ab1012 |
| Histone H3K9me3 | Rabbit polyclonal | Synthetic peptide within Human Histone H3 aa 1–100 (N-terminal) (tri methyl K9) conjugated to KLH | 1:400 | ab8898 |
| LAMIN B1 | Rabbit polyclonal | Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of mouse Lamin B1 | 1:200 | ab16048, Abcam |
| LAMP2 | Mouse monoclonal | The details of the immunogen for this antibody are not available | 1:500 | 555803, BD PharMingen™ |
| LC3B | Rabbit polyclonal | Peptide derived from within residues 1 - 100 of Human LC3B | 1:100 | ab63817, Abcam |
| 5′methyl-cytosine | Mouse monoclonal | Recognizes 5-methylcytosine in methylated DNA | 1:100 | NA81, Calbiochem |
| p16INK4a | Rabbit polyclonal | Corresponding to C-terminus of human p16 | 1:100 | ab7962, Abcam |
| p-AMPKα1/2 (Thr183/172) | Rabbit polyclonal | Epitope corresponding to phosphorylated Thr172 of AMPKα1 of human origin | 1:50 | sc-101630, Santa Cruz |
| p53(SP5) | Rabbit monoclonal | Recombinant human full-length wild type p53 protein | 1:50 | MA5–14516, Pierce |
| RPA194 | Mouse monoclonal | Raised against amino acids 1–300 of RPA194 of human origin | 1:50 | sc-48385, Santa Cruz |
| SQSTM1 (p62) | Rabbit polyclonal | Epitope corresponding to amino acids 151–440 of SQSTM1 of human origin. | 1:200 | sc-25575, Santa Cruz |
| Ub (FL-76) | Rabbit polyclonal | Epitope corresponding to amino acids 1–76 representing full length Ub of human origin | 1:50 | sc-9133, Santa Cruz |
| VIMENTIN | Rabbit polyclonal | Epitope corresponding to amino acids 1–84 mapping at the N-terminus of Vimentin of human origin | 1:50 | sc-5565, Santa Cruz |