Figure 4.

Nucleosome occupancies at different histone modification domains exhibit changes upon HL-60/S4 differentiation with RA or TPA. Panels: (A) H3K4me3; (B) H3K4me1; (C) H3K9ac; (D) H3K27ac. The largest changes occur in H3K4me3 domains, indicating major nucleosome loss at promoters upon HL-60/S4 differentiation (consistent with Fig. 1A and B). Coordinates of H3K4me1, H3K9ac and H3K27ac domains are obtained from the corresponding ChIP-seq experiments performed in this study. Coordinates of H3K4me3 domains are based upon ENCODE ChIP-seq data on undifferentiated HL-60 cells; nucleosome occupancy was calculated from present data on undifferentiated and differentiated HL-60/S4 cells. Plot line colors: O (undifferentiated), black; RA treated, red; TPA treated, blue.