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. 2017 Mar 21;39(5):1206–1214. doi: 10.3892/ijmm.2017.2928

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Copyright: © Ye et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Western blot analysis showed that anterior gradient protein 2 homologue (AGR2) inhibited the decreased expression of tight junction (TJ) proteins (A) zonula occludens-1 (ZO-1), (B) claudin-1 and (C) occludin induced by TNF-α. The results are presented as the mean ± SD. ^**P<0.01 compared with controls; ^#P<0.05 compared with rhTNF-α-treated cells; ^##P<0.01 compared with rhTNF-α-treated cells. Immunofluorescence staining shows that AGR2 inhibited the rhTNF-α-induced abnormal distribution of (D) occludin and (E) ZO-1 proteins.