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miR-486 can adjust the osteosarcoma cell proliferation and metastasis by targeting PKC. (A) After co-transfecting miR-486 and PKC, MG63 and U2OS cell proliferation was detected by MTT assay. Data are shown as mean ± SEM. (B–E) After co transfecting miR-486 and PKC in MG63 and U2OS cells, Transwell assay with or without Matrigel was performed. (F) After co-transfecting miR-486 and PKC, western blot analysis was used to show the protein dynamic changes in expression levels.
