Figure 2.

Representative immunofluorescent images at different cancer stages. Images show different cell sizes and shapes detected in two representative samples from each cancer stage. Collected cells were stained with DAPI (blue staining) and antibodies against CD45 (red staining) and EpCAM (green staining). Leukocytes (bottom row) were stained as a control for DAPI- and CD45-positive cells. Magnification, ×400. Merge, merged images; CD45, protein tyrosine phosphatase, receptor type C; EpCAM, epithelial cell adhesion molecule.