Figure 4.

Influence of exogenous PUMA on Bcl-2 and Bax protein expression in human prostate cancer PC-3 cells. (A) Exogenous PUMA expression, and alterations in Bax or Bcl-2 protein expression, in control and p53-deficient PC-3 cells were assessed by western blot analysis, respectively, with anti-HA, anti-Bax or anti-Bcl-2 antibodies. (B) Bax/Bcl-2 ratios were counted by the quantitation of Bax protein blots divided by the quantitation of Bcl-2 protein blots. **P<0.01 vs. pCET4 empty vector-transfected control cells. Data are presented as the mean ± standard error. PUMA, p53 upregulated modulator of apoptosis; Bcl-2, B-cell lymphoma 2; Bax, bcl-2-like protein 4; HA, hemagglutinin; sc, control; siRNA, small interfering RNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; sip53; siRNA duplexes.