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. 2016 Dec 23;13(3):1281–1287. doi: 10.3892/ol.2016.5532

Figure 2.

Figure 2.

AMPD3 and KIT regulate the expression of each other. (A) The protein expression of KIT in various cancer cell lines was examined by western blotting. (B) The mRNA expression levels of AMPD3 in the different cell lines were evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). (C) GIST-T1 cells were transfected with siCtrl, siKIT-1 or siKIT-2. After 72 h, the mRNA expression levels of KIT and AMPD3 were measured by RT-qPCR. (D) The mRNA expression levels of AMPD3 and KIT in GIST-T1 cells transfected with siCtrl, siAMPD3-1 or siAMPD3-2 were evaluated by RT-qPCR. (E) The protein expression of KIT in AMPD3-depleted GIST-T1 cells was confirmed by western blotting. AMPD, adenosine monophosphate deaminase; siCtrl, control-small interfering RNA (siRNA); siKIT, siRNA targeting KIT; siAMPD3, siRNA targeting AMPD3.