Figure 3.

Sequential treatment with Bix increases tumor metastasis in nude BALB/c mice. (A) U251 cells (2×10⁶) were injected subcutaneously into nude mice (n=9). A total of 4 weeks following injection, lung tissue of sacrificed mice was visualized. Representative images comparing lung tissues between untreated cells and SBT/SeBT cells (left panel). The graph indicates the number of metastatic lung nodules in single or sequential Bix injected mice (right panel). (B) Representative images (hematoxylin and eosin stain; magnification, ×200) show histological changes in the lung tissue and block arrows represent the lung nodules. Bix, BIX01294; SBT, single treatment of Bix; SeBT, sequential treatment of Bix; C, control.