Figure 3.

MMP9 induces cell proliferation in glioblastomas. (A) U87 glioblastoma cell lines were stably transfected with pIRES2-EGFP-MMP9 or pIRES2-EGFP. Cells were cultured and cell proliferation was assessed using a methylthiazolyldiphenyl tetrazolium assay. (B) Colony forming assay. Cells were cultured in soft agar, and the formation of colonies was observed and compared (**P<0.01 and ***P<0.001; n=5). MMP9, matrix metalloproteinase 9; OD, optical density; EGFP, enhanced green fluorescent protein.