Table II.
Restoration of chemosensitivity following MRP1 knockdown.
| A, RNAi knockdown of MRP1 resensitized 5-FU-resistant CRC cells to 5-FU and l-OHP. | ||||
|---|---|---|---|---|
| Cell line | IC50 5-FU [mean ± SD (mg/l)] | RI | IC50 l-OHP [mean ± SD (mg/l)] | RI |
| HCT-8/5-FU | 0.2657±0.003 | 0.23 | 0.1134±0.005 | 0.10 |
| HCT-8/5-FU/ShMRP1 | 0.0608±0.033 | 0.0116±0.002 | ||
| Colo205/5-FU | 0.1181±0.002 | 0.09 | 0.1101±0.062 | 0.15 |
| Colo205/5-FU/ShMRP1 | 0.0108±0.006 | 0.0169±0.022 | ||
| B, RNAi knockdown of MRP1 reversed 5-FU sensitivity | ||||
| Cell line | IC50 5-FU [mean ± SD (mg/l)] | RI | IC50 l-OHP [mean ± SD (mg/l)] | RI |
| HCT-8 | 0.0106±0.004 | 5.73 | 0.0066±0.008 | 1.63 |
| HCT-8/5-FU/ShMRP1 | 0.0608±0.033 | 0.0108±0.006 | ||
| Colo205 | 0.0063±0.004 | 1.84 | 0.0097±0.004 | 1.74 |
| Colo205/5-FU/ShMRP1 | 0.0116±0.002 | 0.0169±0.022 | ||
n=3/group. MRP1, multidrug resistance associated protein 1; RNAi, RNA interference; 5-FU, 5-fluorouracil; l-OHP, oxaliplatin; IC50, half maximal inhibitory concentration; RI, resistance index.