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. 2017 Jan 17;13(3):1797–1806. doi: 10.3892/ol.2017.5604

Figure 4.

Figure 4.

TRIM24 regulates the Wnt/β-catenin signaling pathway. (A) Representative images of GC tissues showing concordant positive staining of TRIM24 and β-catenin in the same sample. (B) Quantitative analysis of expression levles of TRIM24 and β-catenin in 90 GC samples. (C) mRNA expression levels of TRIM24, β-catenin, c-Myc and cyclinD1 in GC cells transfected with NC or TRIM24 siRNA were analyzed using reverse transcription-quantitative polymerase chain reaction analysis, with GAPDH used as an internal control. (D) Protein expression levels of TRIM24, β-catenin, c-Myc and cyclinD1 in GC cells transfected with NC or TRIM24 siRNA, as determined using western blot analysis. β-actin was used as internal loading control. (E) Immunofluorescence staining of subcellular localization of β-catenin in cells. (F) Protein levels of TRIM24, β-catenin, c-Myc and cyclinD1 in GC cells transfected with NC or TRIM24 siRNA (+/− 20 mmol/l LiCl for 24 h), using western blot analysis. Data are presented as the mean ± standard deviation of three independent experiments.*P<0.05; **P<0.01. TRIM24, tripartite motif-containing 24; GC, gastric cancer; siRNA, small interfering RNA; NC, negative control.