Table I.
Primers used for PCR and RT-PCR.
| Name | Sequence, 5′-3′ |
|---|---|
| Construct | |
| E4Bam.F | GTTAGGGATCCGTTTTCCAGGTACCTATCCAAG |
| E7Xho.R | ATTACCTCGAGCGTGCTAGCAGACACTCAGTC |
| E5-M1.F | CTGACCCTGTGAGGCTCCAACTTC |
| E5-M1.R | GAAGTTGGAGCCTCACAGGGTCAG |
| E5-M2.F | TCTGGTGCCTTAGGGAACCC |
| E5-M2.R | GGGTTCCCTAAGGCACCAGA |
| E6Tins.F | GAGGCTTCTCTTTTCATGGTG |
| E6Tins.R | CACCATGAAAAGAGAAGCCTC |
| E7Gdel.F | TCTTAGGAGCCATGCTGATAG |
| E7Gdel.R | TATCAGCATGGCTCCTAAGAG |
| E6Ains.F | CCACCGGGCAAAGCACTTCC |
| E6Ains.R | GGAAGTGCTTTGCCCGGTGG |
| E7Tdel.F | CAACCCCTCTTGGCCCACGG |
| E7Tdel.R | CCGTGGGCCAAGAGGGGTTG |
| RT-PCR | |
| Exon4.F | GTTTTCCAGGTACCTATCCAAG |
| pcDNA.R | TCCACCACCCTGTTGCTGTA |
PCR, polymerase chain reaction; RT, reverse transcription; F, forward; R, reverse.