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. 2017 Feb 22;13(4):2607–2614. doi: 10.3892/ol.2017.5761

Figure 4.

Figure 4.

Regulatory role of the PI3K/Akt signaling pathway on PKM2 expression in MCF-7 cells. (A) The effect of PI3K/Akt signaling pathway on PKM2 protein expression. LY294002, a specific chemical inhibitor of PI3K, downregulated PKM2 protein expression in a dose-dependent manner, and insulin, a potent activator of the PI3K/Akt signaling pathway, increased PKM2 protein expression. (B) The expression of Akt, p-Akt and PKM2 relative to β-actin. *P<0.05, **P<0.01 vs. the untreated control group. (C) The effect of the PI3K/Akt signaling pathway on PKM2 mRNA expression. Quantitative polymerase chain reaction analysis results demonstrated that the inhibition of PI3K by LY294002 did not decrease PKM2 mRNA expression. Furthermore, activation of Akt by insulin did not increase PKM2 mRNA expression. PKM2, pyruvate kinase isoenzyme M2; p-Akt, phosphorylated Akt serine/threonine kinase; PI3K, phosphoinositide 3-kinase.