Table I.
Comparison between the different protocols used for the activation profile and cytokine production of monocyte-derived dendritic cells derived from cervical intraepithelial lesions patients.
| A, Gate (%) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Protocol I | Protocol II | Protocol III | ||||||||
| Cytokine | Control | LSIL | HSIL | CC | Control | LSIL | HSIL | Control | LSIL | HSIL |
| CD11c | 42.65 | 30.91 | 39.54 | 33.87 | 2.19a | 1.57a | 7.26b,c | 30.17 | 46.36b,c | 32.76 |
| HLA-DR | 6.96 | 5.75 | 5.65 | 1.59c | 0.70a | 0.62b | 1.76 | 6.60 | 6.95 | 4.23 |
| CD86 | 3.25 | 2.05 | 2.56 | 1.12 | 0.57b | 0.67b | 1.11 | 1.91 | 1.59 | 3.44 |
| B, Cytokine production (pg/ml) | ||||||||||
| Protocol I | Protocol II | Protocol III | ||||||||
| Cytokine | Control | LSIL | HSIL | CC | Control | LSIL | HSIL | Control | LSIL | HSIL |
| IL-4 | 26.68 | 34.97 | 29.95 | 31.30 | 330.60b | 47.31b | 39.16 | 19.43 | 13.02b | 11.34b |
| IL-12 | 272.80 | 271.50 | 277.20 | 244.60 | 42.77a | 105.00 | 84.21 | 544.00b | 1,059.00b | 641.00 |
| TGF-β | 260.20 | 334.50 | 526.50 | 479.30 | 688.30b | 721.90b | 626.10 | 1,602.00a | 4,525.00a | 3,254.00b |
a
P<0.01
b
P<0.05 (comparison between protocols)
c
P<0.05 (comparison between patient groups). Results are expressed as the median (Mann-Whitney test for comparison of paired groups; Kruskal-Wallis and post hoc Dunn's tests for comparisons among three or more groups). CD, cluster of differentiation; HLA-DR, human leukocyte antigen-antigen D related; IL, interleukin; TGF, transforming growth factor; LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion; CC, cervical cancer.