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. Author manuscript; available in PMC: 2017 Apr 24.
Published in final edited form as: Nano Lett. 2016 Mar 23;16(4):2198–2204. doi: 10.1021/acs.nanolett.5b04364

Figure 2.

Figure 2

IMP compatibility with various imaging microscopy. A. 3D perspective of grating geometry; 50% duty cycle and the pitch varies from 500 to 3000 nm; B. The cells spread along the direction of the grating (vertical) within the first hour; C. The cells stop spreading and start contracting after 4 h; D. Aspect ratio of T cells activated on grating and flat PDMS; E. 3D perspective of grid, grid geometry is a square lattice of trenches on a 750 nm pitch, trench width is 100–300 nm; F–G. High-resolution confocal imaging of actin in T cells on 100 and 300 nm grid geometries; H–J. High-resolution imaging of colorized scanning electron micrograph (SEM) of T cells on grid geometries; K. T cell spreading area on grid geometries.