BSGLWE inhibits tumor growth in HCT116 xenograft nude mice. (A) Representative image of the average tumor volume from control, 150 mg/ml, 300 mg/ml and 5-FU groups. (B) Average final tumor weight of each group. (C) Growth curve of tumor volume in nude mice. (D) Effect of BSGLWE and 5-FU treatment on body weight of nude mice. (E) Expression of cell cycle regulatory genes in xenograft tumors as determined by qRT-PCR. (F) Quantification of the mRNA levels of apoptosis-associated genes treatment with different doses of BSGLWE in xenograft tumors as determined by qRT-PCR. (G–I). Expression of PARP, NAG-1, and Foxo3a in xenograft tumor at protein levels as determined by western blotting. Densitometry analysis is presented; and β-actin was used as internal control. (J) Concentration of NAG-1 in serum of nude mice was detected by ELISA assay, data were normalized by tumor weights. Data are presented as mean ± SE of three independent experiments, *p<0.05; **p<0.01, as compared with control group.