Figure 3.

Oridonin treatment induces alterations in the expression of cell cycle regulators. (A) PC3 and (B) DU145 cells were treated with various concentrations of oridonin or DMSO for 24 h, and were then collected and subjected to western blot analysis. β-actin was used as a protein-loading control. The representative western blotting figures (left) and the densitometric analysis (right) of the expression levels of p53, p21 and CDK1 are presented. *P<0.05, **P<0.01 vs. the DMSO control group. DMSO, dimethyl sulfoxide; CDK1, cyclin-dependent kinase 1.