Figure 5.

Effects of oridonin treatment on apoptotic protein and caspase family protein expression. (A) PC3 and (B) DU145 cells were treated with various concentrations of oridonin or DMSO for 24 h, and were then collected and subjected to western blot analysis. β-actin was used as a protein-loading control. The representative figures (left) and the densitometric analysis (right) of the expression levels of Bcl-2, Bax, cleaved caspase-3, −9 and PARP are presented. *P<0.05, **P<0.01 vs. the DMSO control group. DMSO, dimethyl sulfoxide; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; PARP, poly (ADP-ribose) polymerase.