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. 2017 Jan 10;13(3):1379–1385. doi: 10.3892/ol.2017.5581

Figure 1.

Figure 1.

RASAL2 expression in CRC tissue samples and cell lines. RASAL2 expression was assessed using IHC staining of a human CRC tissue microarray. Relative RASAL2 protein expression in (A) grade 1–3 and (B) stage I–IV CRC tissue compared with wild-type colorectal tissue. (C) Representative images from the IHC stained tissue microarray. (D) RASAL2 mRNA expression in wild-type, cancerous and metastatic colorectal tissue samples relative to β-actin. RASAL2 (E) mRNA and (F) protein expression in the CRC cell lines SW620, LoVo and HCT116, relative to the wild-type colorectal cell line, NCM460. Values are presented as the mean ± standard deviation of triplicate data. *P<0.05, **P<0.01, ***P<0.001 vs. the wild-type tissue or cell line. RASAL2, RAS protein activator-like 2; CRC, colorectal carcinoma; IHC, immunohistochemistry.