RASAL2 is involved in the motility and invasion of LoVo and HCT116 cells. (A) The cell invasion assay demonstrated that RASAL2 knockdown significantly promoted cell invasion. (B) The wound healing assay demonstrated that RASAL2 knockdown promoted cell migration. (C) The cell adhesion assay demonstrated that RASAL2 knockdown significantly promoted cell adherence. Values are presented as the mean ± standard deviation of triplicate data. **P<0.01, ***P<0.001 vs. the Con cells. RASAL2, RAS protein activator-like 2; Con, untransfected; Ct, control; shRNA, short hairpin RNA; OD, optical density.