Figure 4.
Effect of melatonin treatment on the expression of p65, p38 and JNK/MAPK proteins in SGC7901 cells. (A) Western blot analysis for p65 and p-p65 following treatment with 2 mM melatonin for 0–48 h. (B) The expression levels of p-p65/p65 proteins were quantified by densitometric analysis using the control as 100%. (C) Altered nuclear translocation of NF-κB p65 following 2 mM melatonin treatment for 24 h. Following cell fixation, nuclei were visualized by blue DAPI staining, and nuclear NF-κB translocation was observed by overlaying with anti-p65 green immunofluorescence microscopy. (D) Western blot analysis for p38, p-p38, JNK and p-JNK following melatonin treatment. *P<0.05, **P<0.01 vs. the control group. p-, phosphorylated; JNK, c-Jun-N-terminal kinase.