Figure 4.

Psoriasin and invasion of pancreatic cancer cell lines. The invasion of MIA-PaCa-2 (A) and PANC-1 (B) cell lines were determined using a Transwell invasion assay in triplicates. (C) Expression of MMP-2, p27^Kip1, cyclin D2 and caspase-3 was detected in MIA-PaCa-2 cells with differential expression of Psoriasin using conventional PCR. (D) Active MMP-2 and MMP-9 were determined using gelatine zymography. Invasion rates were calculated against corresponding control in which the same number of cells were seeded at beginning of the experiments. Three independent experiments were performed. ^*p<0.05, ^**p<0.01.