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. 2017 Jan 17;13(3):1745–1752. doi: 10.3892/ol.2017.5601

Figure 2.

Figure 2.

(A) PCR product for the VEGF165 coding sequence (611 bp). (B) Linearized pLVX-EGFP-3FLAG plasmid upon digestion with EcoRI (7.7 kb). (C) The pLVX-VEGF165-EGFP-3FLAG recombinant expression vector was identified in bacterial clones by PCR. Lane 1, blank control group; lane 2, negative control group (LV-EGFP vector, 252 bp); lane 3, DL2000 DNA Marker; lanes 4–11: eight pLVX-VEGF165-EGFP-3FLAG-transformed clones, of which, clones 6, 8 and 11 were positive clones (837-bp band). M, marker; LV, lentivirus vector; VEGF, vascular endothelial growth factor; EGFP, enhanced green fluorescent protein; PCR, polymerase chain reaction.