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. 2017 Jan 17;13(3):1745–1752. doi: 10.3892/ol.2017.5601

Figure 5.

Figure 5.

Detection of mRNA and protein levels of VEGF165 in MCF-7 cells following transfection. (A) Polymerase chain reaction analysis of VEGF165 mRNA levels in MCF-7 cells upon transfection. (B) Histogram of VEGF165 mRNA levels in MCF-7 cells. Compared with non-transfected cells and cells transfected with LV-EGFP, cells transfected with LV-VEGF165-EGFP contained significantly higher amounts of VEGF mRNA. Columns indicate means; bars indicate standard error. *P<0.05 vs. control. (C) Western blot analysis of VEGF165-EGFP fusion protein upon transfection. VEGF165-EGFP fusion protein could only be detected in the LV-VEGF165-EGFP group, while normal VEGF could be detected in all the three groups. LV, lentivirus vector; VEGF, vascular endothelial growth factor; EGFP, enhanced green fluorescent protein; mRNA, messenger RNA.