Variant-specific PRMT8 expression in various cancer cell lines. (A) PRMT8 expression was measured by RT-PCR in the human CRL-2073 teratocarcinoma, human U-2OS bone osteosarcoma, adult human Caco-2 colorectal adenocarcinoma and adult human A172 and U87MG glioblastoma cell lines. hESCs were used as a positive control for PRMT8 expression. β-actin was used as a loading control. (B) PRMT8 expression was measured by RT-PCR in (non-tumorigenic) human MCF-10A mammary gland epithelial cells, human SK-BR-3 mammary gland adenocarcinoma cells, and human MDA-MB-231 mammary gland adenocarcinoma cell lines. hESCs were used as a positive control for PRMT8 and PRMT8 variant 1 and, based on the data from 3A, U87MG was used as a positive control for PRMT8 variant 2. PRMT8, protein arginine methyltransferase 8; RT-PCR, reverse transcription-polymerase chain reaction; hESCs, human embryonic stem cells.