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Western blot analysis of PARP, PTEN and Akt expression. PTEN was detected in HEC1-A and KLE cells, furthermore weak bands were observed in AN3CA and RL95-2 cell lines. PARP and Akt were detected in all cell lines, and p-Akt was detected in cell lines without or little PTEN. Lanes: A, AN3CA; H, HEC-1A; K, KLE; and R, RL95-2. PARP, poly (ADP-ribose) polymerase; PTEN, phosphatase and tensin homolog; Akt, AKT serine/threonine kinase; p, phosphorylated.
