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Differential scanning fluorimetry, DSF, curves and their first derivatives for E. coli DHFR in the presence inhibitors belonging to the pyrroloquinazoline and diaminopteridine class of compounds. (A) DSF curves for NSC740, MTX. (B) DSF curves for NSC309401, AMPQD. (C) DSF curves for NSC339578. The Gaussian fit of the first derivatives are given in (D)–(F).
