Figure 4.

Cell proliferation assay. PLC/PRF/5 cells were cultured in 6-well plates (solid line) or as cell spheres (dash line), for 14 days. The cells were seeded onto 96-well plates and cultured in DMEM supplemented with 10% fetal bovine serum, or in HSM for 3 days. The cultured cells were subjected to an MTS assay. Data are presented as the mean ± standard deviation. Error bars, standard deviation; n=4. Experiments were carried out in triplicate, DMEM, Dulbecco's modified Eagle's medium; HSM, hepatocyte selection medium, *P<0.05, as compared with DMEM.