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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Environ Toxicol. 2016 Oct 24;32(8):2004–2020. doi: 10.1002/tox.22374

Figure 3. Arsenite activated Nrf2 in HBE cells.

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Figure 3

Cells were treated with 5 μM, 10 μM and 20 μM arsenite for 24 h or 10 μM arsenite for 6 h, 12 h and 24 h, respectively. The effects of arsenite on Nrf2 protein level at different time points and doses were shown in (a)-(c). The level of Nrf2 in the nucleus of HBE cells treated with arsenite was determined by immunofluorescence (d) and (e). Representative images (400×) of immunofluorescence results were showed in (d). Fluorescence intensity was obtained from Image-Pro Plus software. The mRNA level of Nrf2 in HBE cells was illustrated in (f) and (g). "*" denotes a significant difference (P<0.05) detected between treated and untreated cells.