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. 2017 Jan 26;13(3):1789–1796. doi: 10.3892/ol.2017.5654

Figure 2.

Figure 2.

miR-600 influences the expression of EMT-associated proteins by targeting p53 in mutant p53-expressing human CRC cell lines. (A) Western blotting revealed that overexpression of miR-600 through lentiviral-mediated transduction inhibited p53 protein expression in CRC cell lines SW480, SW620 and DLD-1. (B) The putative miR-600 binding site of the 3′UTR of WT and mut p53 mRNA. Underlining indicates the sites of mutation. (C) Overexpression of miR-600 suppressed the activity of the luciferase reporter containing the 3′ UTR of WT p53, but not the reporter with the 3′ UTR of mut p53. (D) Western blotting demonstrated that overexpression of lentiviral-mediated miR-600 overexpression resulted in increased expression of E-cadherin and β-catenin, and decreased expression of MMP-9. β-actin was used as a protein-loading control. **P<0.01. miR, microRNA; WT, wild-type; mut, mutant; Luc, luciferase control; UTR, untranslated region; MMP-9, matrix metalloproteinase 9.