Figure 3.

TDZ induced caspase-dependent apoptosis and Akt-CDK2 associated cell cycle progression. (A) Decreased expression of apoptosis-associated proteins, including procaspase8, PARP and survivin, in A549-sphere cells treated with TDZ was detected using western blotting. (B) Decreased expression of cell cycle associated proteins, including CDK2, Akt and p-Akt, in A549-sphere cells treated with TDZ was detected using western blotting. All experiments were repeated three times with GAPDH as a loading control. TDZ, thioridazine; PARP, poly ADP-ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CDK2, cyclin-dependent kinase 2; Akt, protein kinase B; p-Akt, phosphorylated-protein kinase B.