Figure 4.

miR-150 negatively regulates gene expression of ROCK1 in OS cell lines. (A) Reverse transcription-quantitative polymerase chain reaction analysis of mRNA expression of ROCK1 in U2OS, MG63 and SaOS2 OS cell lines overexpressing miR150. Student's t-test was used to analyze significant differences. *P<0.05. Data are presented as the mean + standard deviation. (B) Western blot analysis of protein expression of ROCK1 in U2OS, MG63 and SaOS2 OS cell lines overexpressing miR150. A corresponding NC was used in the absence of miR-150. β-actin protein was used as an additional control. miR, microRNA; ROCK1, Rho-associated kinase 1; NC, negative control.