Figure 4.

Akt inhibitor LY294002 restores the chemosensitivity of CYFIP2-deficient SGC7901 cells to 5-FU. (A) Western blot analysis of p-Akt, Akt, active caspase-3 and cleaved PARP protein expression SGC7901 cells following CYFIP2 knockdown and treatment with 5-FU. Quantification of (B) p-Akt, (C) cleaved caspase-3 and (D) cleaved PARP protein bands. Values were normalized to β-actin. (E) SGC7901 cell apoptosis was quantified using flow cytometric analysis following treatment with LY294002. Results are presented as the mean ± standard deviation (n=3). Akt, Akt serine/threonine kinase 1; CYFIP2, cytoplasmic fragile X mental retardation 1-interacting protein 2; 5-FU, 5-fluorouracil; p, phosphorylated; PARP, poly (ADP-ribose) polymerase; shRNA, short hairpin RNA; FL, fluorescence; A, area; PI, propidium iodide; FITC, fluorescein isothiocyanate.