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. 2017 Apr 13;7(2):200–205. doi: 10.1016/j.ijpddr.2017.04.002

Fig. 2.

Fig. 2

The optimization of the conditions of the assay using CellTiter-Glo®reagent. (A) The correlation between the number of viable T. equiperdum IVM-t1 and luminescence was measured using the following optimized conditions: 25 μL of reagent at 5 min after mixing. All of the plots were calculated from four independent experiments and are shown as the mean luminescence signal ± standard deviation. The scale of the X and Y axes is log-10. (B) The optimization of the initial concentration for T. equiperdum IVM-t1 culture using 96-well cell culture plates. Each plot was calculated from three independent experiments and shows the mean value ± standard deviation. (C) The optimization of the DMSO concentration in the medium. All bars were calculated from three independent experiments and are shown as the mean survival rate ± standard deviation. Student's t-test was used to analyze the differences between 0% DMSO (negative control) and each of DMSO concentrations. ***p < 0.001.