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. 2017 Apr 25;8:710. doi: 10.3389/fmicb.2017.00710

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Copyright © 2017 Ang, Zainul Rahim, de Sessions, Lin, Koh, Pethe, Hibberd and Alonso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro growth kinetics of mshA KO and mshA ethA/R (m/e) double KO mutants. Growth kinetics of Erdman (A,D), H37Rv (B,E), CDC1551 (C,F) parental, mshA KO and mshA/ethA/R (m/e) double KO strains in 7H9 media supplemented with ADS (A–C) or OADC (D–F) over a period of 14 days. Every 2–3 days, OD₆₀₀ was measured. The experiment was repeated twice independently.