Table 3.
MIC50 of INH and ETH on mshA single KO and mshA ethA/R double KO mutants.
| Strain | MIC50 INH | MIC50 ETH |
|---|---|---|
| CDC1551 | 0.26 | 7.18 |
| CDC1551 ethA/R KO | 0.2 | 31.04 |
| CDC1551 ethA/R KO complement ethA/R | 0.29 | 5.12 |
| CDC1551 mshA KO | 0.98 | NA |
| CDC1551 mshA KO complement mshA | 0.43 | 11.82 |
| CDC1551 m/e KO | 0.57 | NA |
| CDC1551 m/e KO complement m/e | 0.30 | 4.76 |
| Erdman | 0.18 | 3.04 |
| Erdman ethA/R KO | 0.18 | 19.69 |
| Erdman ethA/R KO complement ethA/R | 0.17 | 2.67 |
| Erdman mshA KO | 0.49 | 20.63 |
| Erdman mshA complement mshA | 0.28 | 5.42 |
| Erdman m/e KO | 0.37 | NA |
| Erdman m/e KO complement m/e | 0.20 | 13.54 |
| H37Rv | 0.20 | 3.30 |
| H37Rv ethA/R KO | 0.28 | 12.55 |
| H37Rv ethA/R KO complement ethA/R | 0.23 | 1.99 |
| H37Rv mshA KO | 0.85 | 40.67 |
| H37Rv mshA KO complement mshA | 0.28 | 12.89 |
| H37Rv m/e KO | 0.80 | NA |
| H37Rv m/e KO complement m/e | 0.27 | 11.12 |
Drug susceptibility assays were performed in 7H9-OADC. MIC50 are indicated in μM. NA, not available due to complete ETH resistance.