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. 2017 Apr 25;8:684. doi: 10.3389/fmicb.2017.00684

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Copyright © 2017 Stojkovic, Szijártó, Kaszowska, Niedziela, Hartl, Nagy and Lukasiewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural analysis of lipopolysaccharides from K. pneumoniae strains: (A) Kp24 (gml+), (B) Kp4 (gml–), (C) Kp4 transformed with pKP100 (recombinant plasmid carrying gmlABC), and (D) Kp4 transformed with pSU2718 (empty cloning vector). Anomeric regions of ¹H, ¹³C HSQC NMR spectra overlaid with ¹H NMR profiles of the isolated O-PS for strains Kp24 (A), Kp4 (B), Kp4/pKP100 (C), and intact LPS for the strain Kp4/pSU2718 (D) (left panels). NMR spectra for the strain Kp4/pSU2718 were acquired using HR-MAS technique at a spin rate of 4 kHz at 30°C. The capital letters refer to carbohydrate residues as described in Tables 1, 2. For each strain O-PS gal-I and gal-II repeating units were shown as inset structures (right panels).