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. 2017 Apr 25;8:684. doi: 10.3389/fmicb.2017.00684

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Copyright © 2017 Stojkovic, Szijártó, Kaszowska, Niedziela, Hartl, Nagy and Lukasiewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot reactivity of isogenic gml mutants of K. pneumoniae O1 strain. The wild-type (gml–) strain Kp4 was trans-complemented with the cloned gml operon or the empty vector (as control). Separated LPS molecules were reacted with monoclonal antibodies specific to different galactan structures as shown: (A) anti-gal-II mAb 5C10, (B) anti-gal-I mAb 1F11, (C) anti-gal-III mAb 5A4. Serotype O2 (PCM-27) and O3 (PCM-11) strains were used as controls (lanes 1 and 2). Lane description: 1, PCM-27 (O2gml+), 2, PCM-11 (O3, control), 3, Kp4 (O1gml–), 4, Kp4/pSU2718 (O1gml–), 5, Kp4/pKP100 (O1gml+).