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. 2017 Apr 25;8:447. doi: 10.3389/fimmu.2017.00447

Figure 1.

Figure 1

Bronchial epithelial cell-conditioned media (BEC-CM) enhances the expression of CD141/CD123/DC-SIGN on monocytes. (A) Phenotypic analysis of surface markers on monocytes cultured withBEC-CM. FACS analysis was performed on monocytes that were cultured for 48 h with medium alone or with BEC-CM. Representative histograms (thick lines) of the indicated antibody staining are plotted with the corresponding isotype controls (shaded histograms). All data were obtained with similar results in at least four independent experiments. (B–D) Dendritic cells (DCs) were obtained by culturing monocytes with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL). Then, monocytes and DCs were cultured for 48 h with medium alone or with BEC-CM. Bar histograms of the mean fluorescence intensity (MFI) ratio calculated with its specific isotype for (B) CD141, (C) CD123, and (D) DC-SIGN. Data are expressed as means ± SEM of at least eight independent experiments for monocytes and at least five independent experiments for DCs. Statistical analysis was performed using analysis of variance test (ANOVA) (followed by Tukey multiple comparisons test) (*P < 0.05, **P < 0.01). (E–G) Monocytes were cultured for 48 h with BEC-CM, PNEC-CM, or PAEC-CM or with the corresponding control media 1:LHC-8, 2: PneumaCult™-ALI, 3: CELLnTEC. Data represent the MFI ratio calculated with its specific isotype for CD141 (E), CD123 (F), and DC-SIGN (G). Data are expressed as means ± SEM of at least five independent experiments. Statistical analysis was performed using ANOVA (followed by Tukey multiple comparisons test), *P < 0.05, **P < 0.01.